가스크로마토그라피-염광광도검출기를 이용한 지방산의 신속간편한 정량

A Rapid and Convenient Method for the Determination of Fatty Acid by Gas Chromatography-Flame Photometric Detector

A new method of determination of fatty acid using gas chromatography-flame photometric detector (GC-FPD) is described. Fatty acid was methylthiomethyl-esterified with methylthiomethyl chloride in 1,8-diazabicyclo[5,4,O]-undec-7-ene catalyst and its concentration measured by GC-FPD with 3% OV-1 or 3% FS-1 column. The FPD responses of thirteen fatty acid methylthiomethyl esters were examined and were proportional to the concentration of the esters without regard to their chemical structures. Consequently it was possible to determine various fatty acids using one standard calibration curve by GC-FPD. We could rapidly and conveniently determine 13 fatty acids in Ginseng Radix alba by this method.