GC-FID에 의한 Rat 뇨중 Tranylcypromine의 분석

GC-FID Analysis of Tranylcypromine in Rat Urine

A gas chromatography with flame ionization detection was developed to measure tranylcypromine in rat urine. The method involves extraction of the drug and the internal standard, N-methyl-3-phenylpropylamine from the urine using ethyl acetate and back extraction into 0.5N H2SO4. Following final extraction using dichloromethane, both the drug and the internal standard were converted to trifluoroacetyl derivatives and analyzed using a column of 3% SE-30 on 80/100 mesh Chromosorb W(HP). A calibration curve was constructed in the range of 5~50mcg tranylcypromine sulfate in 0.5ml urine and found to be linear. The detection limit was 2mcg. The tranylcypromine could be analyzed with the percent recovery of 100.81 +/- 8.13 (SD) in a concentration range of 8~40mcg in 0.5ml urine. When 0.4mmol/kg dose of the drug was administered through an oral route, excretion percent of tranylcypromine in rat urine over 36 hr was found to be 11.90 +/- 6.04 (SD) for tranylcypromine sulfate and 2.23 +/- 0.63 (SD) for benzyl trans-2-phenylcyclopropanecarbamate.