2-Bromoacetyltriphenylene 유도체화제를 이용한 카르복실기 함유성분의 분석법(I) 프로스타글란딘 E2와 F2alpha 혼합물의 HPLC에 의한 분리정량

UV-HPLC Determination of Carboxyl Group Using 2-Bromoacetyltriphenylene as a Pre-labeling Reagent The isolative determination of prostaglandin E2 and F2alpha by HPLC

A new UV labeling reagent was developed and used in HPLC for the determination of prostaglandin E2 which have weak UV light-absorbing propertiy. This reagent, 2-bromoacetyltriphenylene, was synthesized by the bromination of 2-acetyltriphenylene which was obtained from triphenylene by Friedel-Crafts reaction. The wave length maximum(lambdamaxCH3CN) of this reagent was 268nm. Prostaglandin E2 was extracted from prostaglandin E2-beta-cyclodextrin using a Sep-pak C18 cartridge. The prostaglandin E2 was labeled with 2-bromoacetyl-triphenylene in acetonitrile using 18-crown-6-ether as catalyst. Derivatized Prostaglandins were separated on a reversed-phase column (Radial-pak) mu-Bondapak C18 using acetonitrile : water=60 : 40 as mobile phase. The effluent was monitored by UV detector at 254nm filter kit. Linearity of calibration curve was obtained between 30ng and 140ng, and the lower limit of detection was 5ng.