인삼 단백질분획에 대한 폴리아크릴아마이드 전기영동

Polyacrylamide Gel Electrophoresis on Ginseng Proteins

Korean ginseng was purified to obtain radioprotective protein fractions by buffer extraction, ammonium sulfate fractionation, CM-cellulose column chromatography, heat inactivation and Sephadex G-75 column chromatography. The final three fractions, GI, GII and GIII were subjected to Disc-polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. The molecular weights (M.W.) of native and denatured proteins were estimated by using regression line equations obtained from the mobilities of standard proteins. As the results, in Disc-PAGE, the GI fraction showed two protein bands with M.W. of above 213,000 and 55,000, GII showed one band with M.W. of 44,000 and GIII, also one band with M.W. of 19,000. In SDS-PAGE, GI fraction gave four subunit bands with M.W. of above 114,000, 27,000, 24,000 and 19,000, GII gave two bands with M.W. of 46,000 and 22,000 and GIII, one band of 19,000.