효모에서 발현된 유전자 재조합 탈메치오닌 인간 성장호르몬의 일반 약리작용

General Pharmacology of Recombinant Human Growth Hormone without N-Terminal Methionine Expressed in Saccharomyces cerevisiae

The general and some other pharmacological actions of growth hormone without N-terminal methionine(rhGH) were investigated in animals. The hormone had no influenced on the central nervous system and on body temperature at a high oral dose of 40IU/kg in animals. It had neither analgesic nor antiepileptic actions at the High doses. In the isolated ileum and trachea of guinea-pig and isolated stomach fundus and uterus of rat, it showed neither contractive nor relaxing effets at a concentration of 1 X 10-3IU/ml in bath, and no inhibitory action at a dose of 1 X 10-3IU/ml against the contractions produced by histamine (5 X 10-5g/ml), serotonin(1 X 10-5g/ml, acetylcholine(1 X 10-5g/ml) and oxytocin(5 X 10-3IU/ml). Furthermore, the intravenous infection of 20IU/kg rhGH had no influences on the normal blood pressure and respiration in rabbits. These negative results in pharmacological profile are thought that the hormone may not elicit serious side effects On the other hand, the rhGH exhibited a weak inhibitory action of glucose tolerance in normal rats, significantly lowered the blood glucose contents in adrenalectomiglucose tolerance in normal rats, significantly lowered the blood glucose contents in adrenalectomized rats 20 min after i.v. administration of 80IU/kg, and showed a significant inhibitory effect on in vitro glycerol release in epinephrine-stimulated epididymal fat pad segments of rats.