생체실료로 부터 미량 디페닐히단토인의 고상추출에 사용될 흡착제의 특성

Characterization of Adsorbents for the Solid-Phase Extraction of Trace Diphenylhydantoin from Biological Samples

The sorption and desorption properties of four different solid adsorbents were evaluated for the trace enrichment of diphenylhydantoin from biological samples. Graphitized carbon black(GCB) gave the highest adsorption coefficient. And among the organic solvents examined, methanol gave the highest desorption coefficient. Using the GCB column, the optimum elution volume of the eluting solvent was evaluated from the breakthrough curve of diphenylhydantoin. The usefulness of GCB as the solid adsorbent was examined for the solid-phase extraction of diphenylhydantoin from serum in the concentration range of 20-50mcg/ml.