alpha-아밀라아제 저해제 생성 방선균, Streptomyces minoensis DMCJ-144의 저해제 생산을 위한 최적 배양 조건

Optimum Culture Conditions for alpha-Amylase Inhibitor Production of Streptomyces minoensis DMCJ-144, a alpha-Amylase Inhibitor Producing Actinomycetes

Streptomyces minoensis DMCJ-144 isolated from soil produces the alpha-amylase inhibitor. Optimum culture conditions for alpha-amylase inhibitor production of the strain were determined in this experiment. The optimum composition of the culture medium was studied by supplementing various carbon sources, nitrogen sources, vitamins, and metal salts to the basal medium containing 1% glucose, 0.1% asparagine, 0.005% MgSO4.7H2O, 0.005% K2HPO4, 0.005% NaCl. Other culture conditions such as the culture temperature, initial pH of the medium, aeration, and culture time were also investigated. When the strain was cultured in a 100ml flask containing 20ml of 2% glucose, 0.5% beef extract, 0.0002% riboflavin, 0.0002% thiamine. HCl, 0.01% ZnCl2, 0.005%MgSO4 .7H2O, 0.005%CuSO4.5H2O, 0.005% K2HPO4, 0.005% NaCl, pH 7.2, 180 rpm at 30oC, the maximum production of the alpha-amylase inhibitor was observed after 5 days of the cultivation.