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생쥐의 장내 미생몰로부터 새로운 슬포트란스훼라제 생산균의 분리

Isolation of Sulfotransferase Producing Bacteria from Mouse Intestinal Microflora

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Haemophilus K-12 producing novel sulfotransferase was isolated from mouce intestinal flora. The enzyme catalyzed the transfer of sulfate group from p-nitrophenylsulfate to phenolic compounds. The optimum medium condition for the sulfotransferase production was 0.2% sucrose, 1% yeast extract, Na2HP0 4 and 0.5% NaCl. The enzyme production was induced by donor substrate, but was not by acceptors. When p-nitrophenylsulfate was used as a donor substrate, 1-naphthol was best substrate, followed by phenol, p-acetaminophenol and tyramine.

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