학술저널
Hydroperoxide 의존성 반응에서의 Cytochrome P-450의 산화활성종 형성양식
Mechanism of Peroxide-supported Hydroxylation by Cytochrome P-450: Its Formation Pattern of the Active Intermediate
- 대한약학회
- 약학회지
- 제37권 제1호 (1993년)
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1993.0295 - 99 (5 pages)
- 2
Peroxidase activity of cytochrome P-450 was examined using N, N-dimethylaniline (NDA) as a substrate and cumene hydroperoxide (CHP) as an oxidant. The initial rates of the N-demethylation for varied concentrations of NDA (0.05-0.5 mM) by P-450 at different fixed concentrations of CHP (0.02-0.2 mM) were determined. The results suggest that P-450 proceeds its peroxidative reaction by the rapid equilibrium random bi bi mechanism to form a ternary complex with substrate and oxidant as an active intermediate.
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