루모갈리온, 슈퍼크롬 가넷 와이 및 알킬 유도체에 의한 염기성 의약품의 형광정량

Fluorophotometric Determination of Basic Drugs with Lumogallion, Superchrome Garnet Y and Their Alkyl Derivatives

Basic drugs could be extracted as ion-paired complexes with Lumogailion, Superchrome Garnet Y and their alkyl derivatives from aqueous acid solution, and then determined fluorometrically after addition of aluminum ion. The analysis was carried out as follows; To a 1ml portion of basic drugs (10-9~2 X 10-8 mole/ml), 1ml of 0.01 w/v% fluorescent reagent solution, and 10ml of dichloroethane are added. The mixture is stirred for 1 minute. After standing for a few minutes, the dichloroethane layer is transfered to 1ml of 0.1w/v% Al(NO3)3 ethanol solution. After mixing, and standing for 30 minutes at room temperature, the fluorescence intensity is measured with each maximum excitation and emission wavelength. The reagent blank is run through the whole procedure. From the degree of enhancement of fluorescence intensity, hexyl and dodecyl lumogailion and Superchrome Garnet Y were judged to be the useful one of fluorescent reagent for basic drugs analysis.