Monodansyl Cadaverine유도체화된 유리지방산류의 H2O2-Bis(2,4,6-trichlorophenyl) Oxalate에 의한 고속액체크로마토그라피

H2O2-Bis(2,4,6-trichlorophenyl) Oxalate Chemiluminescence Detection of Monodansyl Cadaverine Derivatives of Free Fatty Acids in High Performance Liquid Chromatography

The sensitive detection of free fatty acids was investigated by using H2O2-bis(2,4,6-trichlorophenyl) oxalate chemiluminescence system after monodansyl cadaverine labeling. Because dansyl moiety is well excited by this chemiluminescence system, monodansyl cadaverine was a prominent reagent to this system for the determination of free fatty acids. The eluent of 50mM tris-HCl buffer (pH 7.7)-acetonitrile (1 : 4, v/v) was run through TSK gel ODS 80 TM column. The reagent solutions were mixed with the eluent containing the monodansyl cadaverine derivative of fatty acids from the column. By this system, linolic acid was detected 50fmol by injected amount.