Anti-IL-1beta 단일클론 항체를 이용해서 발열환자의 뇨중 IL-1beta inhibitor의 확인

Identification of the IL-1beta inhibitor in the febrile patient urine by anti-IL-1beta monoclonal antibody

To effectively purify of IL-1 inhibitor from human febrile urine, we have established monoclonal antibody that reacts with human recombinant interleukin 1beta(IL-1beta). The antibody, designated ON-1, was highly specific to IL-1beta and no cross-reaction with other cytokines(IL-1alpha and IL-4) was observed. As the results of ELISA inhibition assay and Western blotting method, it was further identified that ON-1 had high binding specificity with IL-1beta. IL-1 receptor binding material from febrile patient urine was effectively purified with affinitv column chromatography which conjugated with ON-1. This urinary material inhibited the thymocyte proliferation in a dose-dependent manner. IL-1beta induced thymocyte proliferation activity was inhibited to 67.3% at 6mcg of the purified urinary material. The result may suggest that this urinary material the purified urinary material. The result may suggest that this urinary material will have antagonic effect on IL-1 action mechanism and act IL-1beta inhibitor.