Brine Shrimp Bioassay를 이용한 해양생물의 세포독성검색

Screening on Cytotoxicity of Marine Organisms Usign Brine Shrimp Bioassay

As a part of chemical study on the bioactive metabolites from marine organisms, we have investigated cytotoxicity using brine shrimp bioassay for each solvent fractions of the marine algae(12 species), marine sponges(3 species), coelenterates(2 species), echinoderms(4 species), marine molluscs(17 species), and ascidians(2 species), respectively. As the results, chloroform extract of Stichopus japonicus (LC50 : 274 mcg/ml), ethyl acetate extract of Anthocidaris crassispina(LC50 : 121mcg/ml), n-butanol extract of Undaria pinnatifida (LC50 : 178mcg/ml), and water extract of Thais clavigera (LC50 : 61mcg/ml) displayed the most significant cytotoxic activity against brine shrimp. Among the marine organisms tested, echinoderms and marine molluscs were thought to be the most active Phylums on screening of new bioactive compounds.