능소화의 꽃받침으로부터 Protein Kinase C저해물질인 Verbascoside의 분리 및 그 생물활성

Isolation and Biological Activity of Verbascoside, A Potent Inhibitor of Protein Kinase C from the Calyx of Campsis grandiflora

The calyx extract of Campsis grandiflora displayed inhibitory activity against protein kinase C from the bovine brain. Separation guided by protein kinase C enzyme assay and bleb forming assay led to isolation of a potent protein kinase C inhibitor that was identified as a known phenylpropanoid glycoside, verbascoside. It suppressed completely bleb-formation of K562 cell surface induced by phorbol 12,13-dibutylate at the concentration of 60mcg/ml and IC50 of the protein kinase C occured at 20mcM. This compound was tested for cytotoxic activity against ten human tumor cell lines in vitro. it exhibited moderate cytotoxic activity against skin tumor cell line M14 (IC50 2.2mcg/ml) and very weak cytotoxicity against other cell lines(IC50>10mcg/ml)