활성산소종에 의한 알데히드 탈수소 효소의 불활성화

Inhibition of Aldehyde Dehydrogenase by the Active Oxygen Species

The susceptibilities of aldehyde dehydrogenase (AIdDH) and alcohol dehydrogenase (ADH) to active oxygen generated by xanthine-xanthine oxidase (XOD) system were studied. Incubation of AIdDH with 2 X 10-3 units of XOD for 30 min at 25oC resulted in the decrease of enzyme activity to 30% and it was inactivated completely when incubated with 5 X 10-3 units of XOD. Whereas 70% of ADH activity was retained after exposure to 5 X 10-3 units of XOD for 30min, 40% of ADH activity was retained after exposure to 5 X 10-2 unit of XOD for 30 min. This inhibition effect by the active oxygen was preventable by catalase and glutathione, but not by SOD. The rates of the NADPH-dependent oxygen consumption by the liver S-9 mixture and microsomes were also determined in this study. Rate of oxygen consumption is increased in the liver S-9 mix and microsomes from phenobarbital-treated rat, and it was consistent with increased lipid peroxidation. In the presense of ethanol as a substrate, the oxygen consumption rates were increased. It is reported that hepatic AIdDH activity is depressed in alcoholic liver diseases, however there is few report that explains the reason of depressed AIdDH activity. These results are supportive of the theory that the increase in hepatic ethanol oxidation through the induced MEOS activity after chronic ethanol feeding generate oxygen radical at elevated rates and it leads to the depression of AIdDH activity.