천련자로부터 분리한 Limonoid 성분의 세포독성

The Cytotoxic Limonoid From the Fruits of Melia Toosendan

The MeOH extract of the fruits of Melia toosendan was selected for futher study by its cytotoxicity and effect on the human breast cancer cell line, MCF-7. The active principle obtained by activity guided fractionation followed by purification gave rise to a needle crystal. The structure was deduced by employing NMR and was determined to be identical with 28-deacetyl sendanin by comparison with published data. This compound induced morphological change of MCF-7 to be rounded with tubule at concentrations between 50mcg/ml and 0.025mcg/ml. This compound, however, showed strong cytotoxic effect on Hepa1c1c7 and HepG2, and their GI50 on the hepatoma cell lines were 0.238mcg/ml and 0.805mcg/ml, respectively. Its effect on lymphocyte of mouse was stronger than hepatoma cell lines, and their ED50 of polyclonal antibody response was 0.011mcg/ml, and ED50 of cell viability was 0.039mcg/ml.