Streptozotocin 유도 당뇨병 랫드의 혈소판 Phospholipase A2 활성에 미치는 Hydroxybrazilin의 영향

Inhibitory Effects of Hydroxybrazilin on the Platelet Phospholipase A2 Activities in Normal and Streptozotocin Induced Diabetic Rats

Platelets play a very important role in normal hemostasis and their functions are more enhanced in various pathogenic states than in normal state. Especially it has been postulated that abnormal platelet and endothelium function might be major factors of microcirculatory disturbance in diabetes mellitus. Hydroxybrazilin, a phenolic constituent of Hematoxylon campechianum has been examined for its inhibitory effect on platelet aggregation. Its antiaggregatory effect might be mediated through the decrease of ATP release from dense granule and those of thromboxane A2 production in normal and streptozotocin induced diabetic rats. The present study was undertaken to gain insight into the mechanism that hydroxybrazilin inhibited thromboxane A2 production in platelets. Thus we measured the effect of hydroxybrazilin on phospholipase A2, a rate limiting step of thromboxane A2 production, in normal and streptozotocin induced diabetic rats. Hydroxybrazilin significantly inhibited the platelet phospholipase A2 activity in normal and streptozotocin induced diabetic rats.