마우스의 혈전증 및 내독소 쇼크 모델에 있어서 Higenamine에 의한 사망율 저하효과

Higenamine Reduced Mortalities in the Mouse Models of Thrombosis and Endotoxic Shock

Higenamine is a tetrahydroisoquinoline alkaloid which was isolated as a cardiotonic principle from Aconiti tuber. I.v. injection of higenamine was reported to increase the cardiac output and heart rate and to decrease the blood pressure and the systemic vascular resistance presumably by stimulating the adrenergic beta-receptors. The anti-platelet and anti-thrombotic effects of higenamine were investigated in this paper. Higenamine(O.5 mg/ml) showed mild inhibitory effect against collagen induced platelet aggregation in vitro and the inhibitory effect was increased with the pre-incubation(5-30 min) of platelet rich plasma(PRP) with higenamine. With the 30 min incubation, the platelet aggregation was almost completely inhibited. And the oral administration of higenamine(50-200 mg/kg) enhanced the survival in the mouse model of thrombosis and that of endotoxic shock. The anti-thrombotic and anti-septic effects of higenamine thus appear to be due to the beta-agonistic and the anti-platelet effects of this compound.