다제내성 황색포도상구균이 가지고 있는 테트라사이클린 내성 플라스미드의 동정

Characterization of Tetracycline Resistance Plasmid of Multidrug-resistant Staphylococcus aureus

The clinical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.44kb plasmid(pKH6) encoded resistance to tetracycline. The cleavage map of pKH6 was determined by restriction enzyme mapping techniques. The cleavage map is given for EcoRV, HindIII, HpaI, HpaII, KpnI and XbaI. Restriction endonucleases BamHI, BglI, BglII, BstEII, EcoRI, HaeIII, PstI, PvuII, SalI, SmaI, and XhoI have no site on this plasmid. The restriction map revealed extensive structural homology between pKH6 and pT181.