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마늘의 조직 센서를 이용한 L-asparagine의 정량

Determination of L-asparagine Using A Garlic Tissue Electrode

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Garlic tissue cells are employed for the conversion of L-asparagine into ammonia. An ammonia gas electrode is used as a detector. The effect of pH, buffer solution, temperature and life time of electrode to have used were investigated in order to optimize the electrode response. The combination of L-asparaginase in garlic tissue cells and the gas electrode response linearly to L-asparagine over the concentration range 1.0X10-4 ~ 1.0X10-1M with a slope of 72.0mV/decade and is selective with respect to other L-amino acids.

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