기체크로마토그래피법에 의한 타액내 유기산의 신속한 스크리닝

Rapid Gas Chromatographic Screening of Saliva Samples for Organic Acids

Rapid gas chromatographic profiling method was applied to saliva from healthy subjects for the analysis of free organic acids. Saliva samples were first saturated with NaHCO3 and extracted with diethyl ether. The aqueous phase was solid-phase extracted using Chromosorb P as the adsorbent and diethyl ether as the eluent after the acidification and NaCl saturation, followed by triethylamine treatment. The resulting triethylammonium salts of acids were directly converted into stable tert.- butyl-dimethylsilyl derivatives, with subsequent analysis by dual-capillary column gas chromatography, and gas chromatograpy-mass spectrometry. From the ten saliva samples studied, twenty eight free organic acids including various fatty acids, hydroxy acids, dicarboxylic acids, and aromatic acids were tentatively identified. Among the acids identified, the concentration of lactic acid was highest for five saliva samples while alpha-hydroxyisocaproic acid was most abundant for one sample, and succinic acid and glycolic acid for two samples, respectively. When the GC profiles were simplified to the corresponding acid retention index spectra of bar graphical form, they presented characteristic patterns for each individual.