Streptomyces aburabiensis SMF30이 생산하는 Cathepsin B 저해물질의 발효생산 및 특성분석

Production and Physico-chemical Properties of Cathepsin B Inhibitor from Streptomyces aburabiensis SMF30

The aim of the present study was to produce low molecular weight cathepsin B inhibitor. A strain of Streptomyces aburabiensis isolated from soil in Korea was selected and the optimum condition for the production of the inhibitor was evaluated. Glucose and soytone were selected as best carbon and nitrogen sources, respectively. From the kinetic analysis in batch fermentation, it was found that the specific cathepsin B inhibitor production rate (qp) was linearly related to specific growh rate (mc). The inhibitor in culture filtrate was purified by adsorption on activated charcoal, butanol extraction, silica gel chromatography, ion exchange chromatography using Dowex-1 (C1- form) and Amberlite IRC-50 (H+ form), and preparative TLC. From the UV, IR, Mass spectroscopy and 1H-NMR. the inhibitor was thought to be a new inhibitor of which molecular weight was 199.