Ginkgolide B 및 ginkgoflavonoids 의 in vitro 와 ex vivo 및 임상에서의 항혈전 작용

Anti-platelet Aggregation Effect of Ginkgolide B and Ginkgoflavonoids, Extracted from Ginkgo biloba, in Vitro, ex Vivo and in Clinic.

The effects of ginkgolides(natural mixture of ginkgolides, ginkgolide B, ginkgolide C) and flavonoids(quercetin, kaempferol. myricetin), extracted from Ginkgo biloba, on ADP and PAF-induced platelet aggregation in vitro and ex vivo were investigated. In these experiments, both of ginkgolides and ginkgoflavonoids did not affect the ADP(5mcM) induced platelet aggregation in vitro. The IC50 value on PAF (0.3mcM) induced platelet aggregation were 2.52mcM (ginkgolide B) and 6.35mcM (natural mixture of ginkgolides) and 2.80mcM (mixture of ginkgolide B and quercetin). Oral administration of ginkgolide B(1 and 3mg/kg) and quercetin(3 and 9mg/kg) to rabbits inhibited ex vivo PAF induced platelet aggregation in a dose-dependent manner. Ginkomin-F tablets administered to the diabetic patients showed inhibitory activities on the ADP and PAF induced platelet aggregation in a dose and time dependent manner.