생체시료로부터 미량 크로바잠의 고상추출을 위한 최적화

Optimization for the Solid-Phase Extraction of Trace Clobazam from Biological Samples

The sorption and desorption properties of four adsorbents were evaluated for the trace enrichment of clobazam from biological samples. Graphitized carbon black(GCB) gave the highest dynamic adsorption coefficient. Among the six organic solvents examined, acetone gave the highest desorption coefficient for the clobazam adsorbed on GCB. Using the GCB colunm, the optimum elution volume of the eluting solvent was evaluated from the on-line monitored breakthrough curve for clobazam. When GCB as the solid adsorbent and acetone as the eluting solvent were used for the solid-phase extraction of clobazam from serum, the recoveries were higher than 83% with good reproducibility in the concentration range of 20-50mcg/ml.