일회통과 관류실험시 물의 수송: 관류액의 종류와 삼투압의 영향

Water Transport during the Single-pass Perfusion Experiments: Effects of Some Perfusates and Their Osmolality

The single-pass perfusion experiments were performed in anesthetized rats to investigate the effects of perfusates and their osmolality on the water transport and to determine the correlation between the extent of water transport and the volume change of perfusate. Phenol red was used as a nonabsorbable marker. In normal rats, when perfused at a flow rate of 0.5ml/min, 2-(N-morpholino) ethanesulfonic acid (MES) and Sorensen''s phosphate buffers showed minimal net water transport as 0.125 and 0.173%/cm of intestinal length, respectively. Hypotonic perfusate of 200mOsm/kg of water and hypertonic perfusate of 400mOsm/kg of water generated significant water transport compared with isotonic perfusate of 300mOsm/kg of water. There was a linear correlation between the extent of water transport and the volume change of perfusate, suggesting that the volume change can be used as a measure of water transport.