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미생물에 의한 Cholesterol과 그 유도체의 분해기전 II 19-Hydroxyaxdrost-4-ene-3,17-dione의 분리

Degradation Mechanism of Cholesterol and Its Derivatives by Microorganisms. II Isolation of 19-Hydroxyandrost-4-ene-3,17-dione

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When 19-hydroxycholesterol acetate was added into CSD-10 in Nutrient Broth or in a mineral salts medium consisting of KH2PO4 (0.1%),K2HPO4(O.1%), NH4NO3(0.1%), MgSO4 (0.02%), CaCl2 (0.002%), and FeCl3(0.005%), a substantial amount of 19-hydroxyandrost-4-ene-3,17-dione was accumulated prior to accumulation of estrone. From this result and all of previous works, a tentative degradation pathway of 19-hydroxycholesterol acetate to estrone by CSD-10 was derived. 19-hydroxyandrost-4-ene-3, 17-dione seems to be an attractive intermediate for the synthesis of 19-norsteroids.

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