항 히스타민 제의 흡광광도정량법

Spectrophotometric Determination of Antihistamines by Using Metal Indicators NN, EBT and Calcon as Color Developing Agents.

The metal indicator, acidic azo dyes NN, EBT and Calcon are utilized to analyse quantitatively chlorpheniramine, tripelennamine and diphenhydramine forming insoluble ion pair in aqueous solution at proper pH values between the acidic azo dyes and the sample molecules, these compexes are extracted by organic polar solvents, and organic layer is determined spectrophotometrically. Generally, the absorption maxima of the complexes are shifted to longer wavelengths compare to the absorption maxima of the dyes themselves. The binding ratio of the ion pair forming complex molecules in chloroform soln. Are as follows; NN-antihistamines (chlorpheniramine, tripelennamine, diphenhydramine) are NN-1 to antihistamine-1, EBT-antihistamines are EBT-2 to antihistamines 1 and Calcon-antihistamines are Calcon-3 to antihistamines-1. These complexes in chloroform soln. Are very stable, and show higher absorbance than the other organic polar solvents. The binding state of complexes were presumed intermolecular hydrogen bond by their infrared spectra. In the mixture solution of three samples, the aqueous phase is buffered at Ph 1.0, and benzene is used to extract ion pair of diphenhydramine EBT complex selectively. At Ph 1.0 of aqueous layer, Calondiphenhydramine complex is also extracted selectively by benzene. However, in this case very small amount of chlorpheniramine-calcon and tripelennamine-calcon complex is extracted with diphenhydramine-calcon simultaneously. The binding state of diphenhydramine-EBT and diphenhydramine-calcon in benzene are same as the complexes in chloroform. But the absorption maxima of the complexes in benzene are shifted to shorter wavelengths than the complexes in chloroform.