Studies on the Colorimetric Determination of Drugs with Phosphomolybdic Acid. I.

A new calorimetric method established for the determination of ampicillin was based on the reduction of phosphomolybdic acid by ampicillin to be resulted in a molybdenum blue which showed a maximal absorbance at 770nm. The pH effects on a molybdenum blue, the reaction time and the stability of a molybdenum blue and the effect of various substances were studied. The color of a molybdenum blue solution was dependent significantly on temperature, but the wavelength shift was not effected by the molybdenum blue.

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