Gas-liquid Chromatography에 의한 Ethambutol의 정량

Determination of Ethambutol by Gas liquid Chromatography

The quantitative analysis of ethambutol.2HCl as well as commercial ethambutol preparations was undertaken by gas-liquid chromatography by finding optimum conditions, such as the use of internal standard, stability of an ethambutol. 2HCl-caffeine standard solution, and the effect of column temperature, N,O-bis-(trimethylsilyl) acetamide [B.S.A.] concentrations and other substances present in the preparations. Under the chromatographic conditions, an ethambutol.2HCl. caffeine standard solution was infected and immediately followed a B.S.A. injection for silylation. The retention time of ethambutol.2HCl was 3 min. 30 sec. and that of caffeine as an internal standard, 9 min. 30 sec. and that of caffeine as an internal standard, 9 min. 30 sec. The relative molar response of ethambutol.2HCl and caffeine studied was 2.08. Ethambutol. 2HCl could by quanitated up to 1x10-8 moles. The possible decomposition of B.S.A. due to the moisture when tested and the incomplete reaction for silylation could be minimized.