흡광도측정법에 이한 황백과 제제 중 프로토베르베린 알칼로이드의 정량

Determination of Protoberberine Alkaloids in Phellodendri Cortex and Preparation by Spectrophotometric Method

The Phellodendri Cortex of phellodendron amurense (Rutaceae) is known to contain a numher of isoquinoline alkaloid, and berberine, palmatine, jateorrhizine, phellodendrine and magnoflorine are the major constituents of protoberberine lkaloids. For the determination of protoberberine alkaloids from Phellodendri Cortex and berberin chloride from the preparation, the new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] complex ion. Samplex were extracted with 0.1 nM hydrochloric acid, potassium bipthalate reagent, thiocyanatocobaltate reagent and 1.2-dichloroethane for 60 min. The absorbance of protoberberine alkaloid complexes in 1.2-dichloroethane solution was meaured at 625nm. Calibration curve for berberine was linear over the concentration range of 0.05~0.30mg/ml 1.2-dichloroethane. The method proved to be rapid, simple and reliable for the determination of protoberberine alkaloids from Phellodendri Cortex and berberine chloride from the preparation.