Bisphenol A의 급성노출이 마우스의 면역기능에 미치는 영향

Effects of Acute Oral Adminstration of Bispenol A on the Immune Function in Mice

In order to investigate the effects of bisphenol A (BPA) on immune system in mice we examined the various immunological parameters. After single oral adminstration of BPA to female ICR mice, the weights of bodies and lymphoid organs, splenic cellularity and hematological parameters were examined on day 2 and 7. Among them WBC and splenic cellularity were slightly decreased on day 2. To assess the effects of BPA on humoral immune responses, splenic IgM plaque forming cell (PFC) and serum IgM level were assayed. When BPA was administered after immunization with SRBC, but not before immunization, IgM level was also decreased on day 4 when high dose (2000mg/kg) of BPA was administrated after injection of OVA-antigen. The indexes of splenocyte proliferation (SP) to concanavalin A (Con A) and bacterial lipopolysaccharide (LPS) were measured in vitro by MTT assay. At low concentration BPA slightly increased splenocyte proliferation but at higher concentration it showed signficant inhibitory effects on cell proliferation Mitogen-stimulated SP was also determined with spleen cells from BPA treated mice. Con A-induced SP was slightly decreased and LPS-induced SP was eapecially inhibited at 1000mg/kg and 2000mg/kg of BPA. These results indicate that BPA is able to acutly evoke humoral and cell mediated immune suppression in mice.