눈꽃동충하초 (Paecilmyces japonica DGUM 32001) 균사배양물의 항암 효과에 관한 유세포분석학적연구

Flow Cytometrical Investigation on Antitumor Activity of Mycelial Culture of Insect-born Fungus Paecilomyces japonica DGUM 32001

Protein-polysaccharide fractions, PJ-3 and PJ-4, were prepared from mucelial culture filtrate of an insect fungus, Paecilomyces Japonica DGUM 32--1, and subjected to a flow cytomerical analysis for their in vivo antitumor and immunomodulating activity in ICR mice. When I.p. injected once daily for seven days at 100mg/kg, PJ-4 exerted a strong antitumor activity, showing the growth inhibition ratio of 85.1% against I.p.implanted sarcoma 180 cells, while PJ-3 showed only a weak activity. Moreover, PJ-4 significantly increased the expression level of CD25 (IL-2R alpha-chain) as well as forward scatter (FSC) values of splenic CD8+ T cells. It is also noteworthy that PJ-4 strongly induced the peritoneal exudate cells in the same experiment. In an in virto study, PJ-4 slightly inhibited the growth of sarcoma 180 sells at toe consentration of 50 concentration of 50ug/ml or higher. These results strongly suggest that PJ-4 might exert its antitumor activity through immunostimulation as well as direct inhibitory activity on the tumor cells.