석이중 오르시놀 정량 및 급성독성

Quantative Analysis of Orcinol and Acute Toxicity of Gyrophora esculenta

In previous study, Gyrophora esculenta showed siginificantly inhibitory effect on alpha-glucosidase in vitro and blood glucose elevation in vivo. In the isolating process of active substance, orcinol was separated from Gyrophora esculenta. Orcinol is kwown to be toxic, therefore, in this study, it was analyzed by the TLC densitometry method for quantitative determination from Gyrophora esculenta. The average amount of orcinol of Gyrophora esculenta was 0.2%. For the purpose of removing orcinol, the water extract of Gyrophora esculenta was sequentially fractionated by organic solvents, and the acute toxicity of each fraction was assedded in mice. Among them, the LD50 of butanol fraction was 1.19g/kg(p.o.) and the weight increase of mice in that group was somewhat retareded.