HPLC 형광검출법에 의한 Glyphosate의 혈중농도 측정

Determination of Glyphosate in Whole Blood by HPLC -fluorescence Detection

A rapid and sensitive method for the determination of glyphosate, a phosphates amino acid herbicide, in whole blood is presented. After removal of protein, the whole blood was purified by using the anion exchange resin (Dowex 1), and derivatized with 9- fluorenylmethyl chloroformate (FMCL). Derivatized glyphosate from blood sample was injected onto a Whatman partisil 10SAX column and separated with 0.1M phosphate buffer (pH 2.5) and acetonitrile (ratio=3:1). The high performance liquid chromatography- fluorescence detection gave the detection limit of 86pg and linearity of 0.9999 in the range of 0.25μg/ml and 25μg/ml. The recoveries of glyphosate added to the blood samples were ranged from 75.3% to 100.4% compared to the samples prepared in water. The derivatized glyphosate was stable at various acidity and temperature. This method has been successfully applied to the blood samples of lethal intoxication with the herbicide glyphosate.