소적혈구로부터 새로운 헝태의 세포질 포스포리팍제 A2의 동정 및 특성규명

Identification and Characterization of a Novel Cytosolic Phospholipase A2 from Bovine Red Blood Cells

A Ca2+_dependent PLA2 activity termed Rpla2, was detected in the cytosol of bovine red blood cells (RBCs). The Rplab was characterized as a similar form to Group IV Cpla2, but different in secteral column chromatographic profiles including an anion exchange column.73 examine whether this Rpla2 is different from the well characterized Rpla2, a quinone derivative, BJ5O, was developed and tested for the inhibitory effect on the two PLA2 enzymes. The rPLA2 activity was inhibited by a quinone derivative (BJ5O) with ID50 of 20μM., but had no effect on the cPLA2, suggesting that rPLA2 may be a novel type of cytosolic PLA2 in RBCs.