C6세포에서 phospholipase A2촬성에 대한 ATP의 작용

The Action of ATP on Phospholipase A2 Activation in C6 Cells

To investigate action of ATP on ischemia-induced brain injury we measured phospholipase A2 activity and nitric oxide (NO) production in C6 cells. ATP alone did not have any influence on phospholipase A2 activity but increased NO production. Glutamate (1 Mm) significantly increased phospholipase A2 activity whereas did not increased NO production. ATP significantly inhibited phospholipase A2 activation induced by 0.1μM A23187, 1 Mm glutamate and 1 Mm H2O2, but did not inhibited 1mM PMA-induced phospholipase A2 activation. From the above results, it is suggested that the action of ATP in C6 cells has dual actions, such as the inhibition of agonist-induced phospholipase A2 activation and the increase of NO production.