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학술저널

천연물로부터의 세포내 효소 활성 조절물질의 탐색 및 기능 연구: 갈근의 알코을 탈수소효소 저해 활성 성분

Studies on the Screening of Bioactive Compound Acting on Intracellular Enzymes from Natural Products and Its Mode of Action : Inhibitory Component of Puerariae Radix on Alcohol Dehydrogenase Activity

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Puerariae Radix is one of the medicinal plants used in oriental medicine for hangover. It has been claimed for several pharmacological effects including anti-alcohol abuse, antidipsotropic activity and anti-alcohol intoxication. In connection with Puerariae Radix effects, an activity-guided purification of active substance on alcohol dehydrogenase (ADH) was carried-out. The most active compound was isolated as puerarin (C21H2OO9), molecular weight 416. Puerarin inhibited ADH noncompetitively against ethanol or NAD+.

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