암유발생쥐에서 리포폴리사카라이드에 의해 유도된 사이토카인의 생산에 미치는 염화아연의 영향

EBTects of Zinc Chloride on the Lipopolysaccharide-induced Production of Cytokines in Tumor-bearing Mice

To determine effects of zinc on lipopolysaccharide (LPS)-induced production of proif1am-matory cytokines and Iymphokines in tumor-bearing ICR mice, this study has been investigated. Zinc chloride (Zn) at doses of 1 mghg was administered orally 30 minutes before I.p. injection of LPS (8 mg/kg) 5 times for 7 days. LPS greatly increased tumor necrosis factor (TNF)-α and interleukin (IL)-1β, in both serum and splenic supernatants compared with those in controls. Howeverl Zn strongly decreased Lpsincreased production of TNF-α and IL-lβ in spleenic supernatants compared with those in controls and insignificantly also reduced in serum. LPS insignificantly decreased IL-2 levels in splenic supernatants compared with those in controls but significantly increased interferon (IFN)-γ. Levels. Zn didn't affect IL-2 production in splenic supernatants compared to controls but significantly enhanced the LPS- decreased production of IL-2. Zn significantly increased IFN-γlevels in splenic supernatants compared to controls and did not affect the LPS-increased production of IFN-γ. These findings suggest that Zn may strongly attenuate the LPS-induced pathogenesis of proinf1ammatory crtokines in tumor-bearing state and significantly up-regulate the LPS-induced function of T cells to produce IL-2 with maintaining normally the LPS-increased levels of IFN-γ.