큰눈물버섯 (Psathyrella velutina)으로부터 분리한 단백다당체 PVP의 항암 ·면역활성

Antitumor Immunomodulatory Activity of PVP A Protein-polysaccharide Fraction Prepared from Wild Mushroom Psathyrella velutina

A protein-polysaccharide fraction of a Korean wild mushroomwas psathyrella velutina, PVP, was prepared and its antitumor immunomodulatory activity was investigated. When PVP was administered once daily for seven days from day 1 to day 7 into male ICR mice implanted with 1 ×105 cells of sarcoma 180 tumor cells into the peritoneum on day 4, it inhibited the growth of sarcoma 180 cells by 92.8%. In XTT assay; PVP also exherted in vitro anti-proliferation activity on U-937, a human monoblastoid cell line, as well as sarcoma 180 cells. PVP showed marked stimulatory activity on the immune system in that it induced the accumulation of PEC (the stimulation index, Sl=4.90 at 100 mgHg), stimulated the BALB/C mouse splenic Iymphocytes to form Iymphoblasts (Sl=5.75 at 100mcg/ml), and upregulated the expression of CD25 molecules. All these results strongly support that PVP exhorts its antitumor activity through stimulation of the immune system as well as antiproliferative activity on the tumor cells.