Chirose hamster ovary 세포에서 발현된 preS2 및 S부위 함유 HBsAg의 항체유발능

Antibody productivity of HBsAg containing both preS2 and S regions expressed in Chinese hamster ovary cells

Many studies have provided evidences that hepatitis B surface antigen (HBsAg) including pres region could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. We established CHO cell lines, H-CHO-2 and IY-CHO-11 expressing high levels of HBsAg containing preS2 and S pretein by stable transfection method. These cell lines expressed the correct size (about 1 kb in length) of HBsAg myNA as eld)ected. The purified protein from the culture supernatants of the clones showed the same sizes as those expressed in native hepatitis B virus (24 kDa, 27 kDa, 34 kDa and 36 kDa). Antibody productivity of CHO-derived HBsAg protein at lower dose challenge was higher than the protein containing S region alone expressed in yeast system. These results indicate that CHO-derived HBsAg protein containing preS2 and S region can be effectively used for a better immune response as a HBV vaccine.