느릅나무의 에틸 아세테이트 추출물에 의한 Melanin생성 효과

Effects of Ethyl Acetate Extract from Ulmus davodoama var. japonica on Melanogenesis

Melanogenesis is a physiological process resulted in the synthesis of melanin pigments, which has a role in protecting skin from the damaging effect of ultra-violet (UV)radiation. The main aim of the present study was to examine the effect of Ulmus davidiana var. japonica (UL) on melanogenesis. Cells were cultured in the presence of various concentrations of Ulmus davidiana var. japonica For 48 h, and there were estimated total melanin contents as a final product and activity tyrosinase, a key enzyme, in melanogenesis. Among the four solvent extracts tested, EtOAc extract mostly increased tyrosinase activity And EtOAc extract increased the melanin contents and tyrosinase activity in a dose-dependent manner, Especially it was observed that 100mcg/ml/ EtOAc extract promotes melanin secretion in B16/F10 melanoma cells by 140% at 48 h treatment and activity of increased by 180% in the presence of same concentration. In conclusion, as for EtOAc extract treatment, there was no effect on the viability of B16/F10 cell, only to stimulate melanization.