Jurkat T 임파구의 세포주기 기전에 미치는 저근백피 ( Ailanthus altissima)의 효과

Ehect of Ailanthus altissima Water Extract on Cell Cycle Control Genes in Jurkat T Lymphocytes

Ailanthus altissima has been used to settle an upset stomach, to alleviate a fever and as an insecticide. We reported that the water extract of A. altissima induced apoptotic cell death in Jurkat T.acute Iymphoblastic leukemia cells. Herel we showed the dose-dependent inhibitions of cell viability by the extract, as measured by cell morphology The cell cycle contro1 genes are considered to play important roles in tumorigenesis. The purpose of the present study is also to investigale the effect ot A. altissima on cell cycle progression and its molecular mechanism in the cells. The level of p21-proleln was Increased after treatment ot the extract, whereas both Bcl-2 and Bax protein levels were not changed. These resu1ts suggest that A. altissima Induces apoptotic cel1 death via p2l- dependent signaling pathway in furkat cells which oelete wlld type p53 checkpoint related gene products tested (cyclin D3, cyclin dependent kinase 4, retinoblastoma E2F1) were decreased In their. Protein levels in a dose-dependent manner after treatment of the extract. Taken together, these resu1ts inulcate that the increase of apoptotlc cell death by A. altissima may be due to the inhibition of cell cycle in Jurkat cells.