황해쑥 물분뵉물의 L1210세포에 대한 세포독성과 항산화효소 활성변화

Cyotoxicity of Water Fraction of Artermisia argyi against L1210 Cells and Antioxidant Enzyme Activities

The water fraction exhibiting anticancer activity was Prepared from 70% methanol extract of Artermisia argyi against by stepwise solvent partioning. This water fraction (5μg/ml concentration) showed a considerable cytotoxicity against leukemic L1210 cells with a maximal value of 92% for 3 days culture. Contrastingly to such substantial anticancer activities the identical fraction showed far low toxicity against normal Iymphocytes than chloroform fraction of Artermisia argyi , mitomycine and 5-fluorouracil at every concentration ranging 0.01 μg/ml ∼10.00μg/ml. The cytotoxicity displayed against L1210 cells by the water fraction of Artermisia argyi was found to be pmportinal to the decrease of viability of L1210 cells. On the other hand, 02- ion generation in L1210 cells appeared to be elevated in accordance to cytotoxicity by the water fraction with concurrent increases of superoxide dismuatse (SOD) and glutathione peroxidase (GPx) which are responsible for the conversion of O2- ion and H2O2 respectively. These findings taken together indicate that the death of L1210 cells by the water fraction of Artermisia argyi may be induced at least in part by the detrimental action of reactive oxygen species (ROS) including 02- in spite of substantial efforts of SOD and GPx to ovecome the attack of ROS.