Genistein이 T lymphocyte의 Apoptosis및 복강 Macrophage의 탐식능에 미치는 영향

Effect of Genistein on Apoptosis of T lymphocyte and Phagocytosis of Peritoneal Macrophage

The effects of genistein on murine thymocytes for inducing apoptotic cell death and phagocytic activity of peritoneal macrophage were studied in vivo. Addition of genistein (10 and 50μM) to cultured thymocytes from BALB/C mice definitely promoted DNA fragmentation. Also, cytofluorometric analysis of these cells demonstrated a reduction in misochondrial transmembrane potential (△Ym). But, repeated administration of genistein (1 mg/mouse/day) to mice for 7 days did not cause any detectable DNA fragmentation. Genistein decreased lucigenin chemiluminescence and engu1fment of fluorescein-conjugated E.coli particles in peritoneal macrophage. These results suggest that genistein induce an apoptosis of thymocyte via reduction in △Ym and decrease phagocytic activity of peritoneal macrophage in vitro.