Myxococcus stipitatus JW150이 생산하는 세포독성 물질의 분리 및 특성

Isolation and Properties of Cytotoxic Antibiotics Produced by Myxococcus stipitatus JW150

Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance (MDR) is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-gly-coprotein. By employing a resistant subline of HCT15 to adriamycin (CLO2), we undertook the screening for agents which were effective to multidrug-resistant cancer cells. As a result, a myxobacterial strain n7150 was selected for study since an activity against CLO2 cells was discovered in the strain. Cytotoxicity-guided of actionation of the culture broth led to the isolation of cystothiazole A and melithlazole F. The producing organism was identified as Myxococcus stipitatus by taxonomic comparison with type strains of Myxococcus sp. As well as its morphological and physiological characteristics. Cystothiazole A and melithiazole F demonstrated potent cytotoxicity against certain human cancer cells with IC50 values ranging from 0.03∼0.72μg/ml. Both compounds were interestingly as active against drug- resistant sublines CLO2 and CP7O as against the corresponding parental cells.