Macrophage의 IL-1β Gene Expression 대한 Lectin-conjugated Ellagitannin의 효과

Effects of pectin-conjugated Ellagitannin on the IL-1β, Gene Expression of Macrophage

Lectin-conjugated praecoxin A, which has developed as a missile antitumor drug, is the one that has conjugated with wheat germ agglutinin (WGA), a kind of carbohydrate- binding protein (lectin) especially bound to melanoma. Praecoxin A is a kind of tannin extracted and purified from plants. Beside this direct antitumor effect as tannins, we have examined an activation of macrophage by lectin-conjugated praecoxin A. We also confirmed the gene expression of IL-1β, both in vitro and in vivo. We added 1,10,100μg/ml of lectin-conjugated praecoxin A, 10μg/ml of pectin,10μg/ml of praecoxin A to normal murine macrophage and analyzed the extracted total RNAs by RT- PCR after 4,8, 12,24 hours. Our data demonstrated that pectin-conjugated praecoxin A increased IL-1β, mRNA expression in a dose dependent manner. However, the effectiveness of lectin-conjugated praecoxln A was not superior to pectin and praecoxin A.