눈꽃동충하초 (Paecitomyces tenuipes DGUM 32001) 균사배양물로부터 분리한 단백다당체 PJ-4의 in vitro 면역촬성

Flow Cytometrical Investigation on in vitro Immunomodulating Activity of Pl-4, a Protein-polysaccharide from Culture Flitrate of Insects-born Fungus Paecilomyces Tfnuipes DGW 32001

In the previous report, we described the in vivo antitumor activity of Pl-4, a protein-polysaccharide fraction prepared from the culture filtra1e of an insect-born fungus, Paecilomres tenuipes DGUM 32001. In the present study we elucidated the immunomodulating activity of PJ-4 on the BALB/C mousse splenic Iymphocytes using flow cytometrical techniques. As a result, PJ-4 was found to stimulate the lymphocytes not only to form Iymphoblasts but also to express CD25 (IL-2 receptor u chain) molecule, which is well known as a T cell activation marker. More Interestingly, its T cell stimulatory activity was more strongly exerted on CD8+ T cells than on CD4+ T cells. All these data suggest that PJ-4 exerts its antitumor activity at least partly through stimulation of T cells which play major roles in the cell-mediated immune system.