착체 생성에 의한 황련 알칼로이드의 분리 및 정량

Isolation and Determination of Alkaloids in Coptis Rhizome by Forming Complex

The Coptis Rhizome is known for containing a number of isoquinoline type alkaloids. Berberine, coptisine and palmatine are the major constituents of alkalolds. The alkaloids were isolated and determined by forming complex from Coptis japonica (Ranunculaceae). For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate(II) complex ion. The absorbance of alkaloidal complex in 1,2- dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Coatis Rhizome was linear over the concentration range of 0.2∼0.3 mg/ml. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Coatis Rhizome.