붉은 서나물 잎 (Erechitites hieracifolia Raf.)에서의 Polyphenoloxidase 활성측정 및 항산화효오 특성분석

Properties of Polyphenoloxidase and Antioxidant Enzymes in the Leaves of Erechitites hieracifolia

Polyphenoloxidase activity (PPO) in the leaves of Erechitites hieracifolia was estimated by Warburg's manometric method. The emzyme was most reactive toward chlorogenic acid followed by caffeic acid. Biethyldithiocarbamate and potassium cyanide were shown powerful inhibition rate to the polyphenoloxidase from the leaves of Erechitites hieracifolia . We confirme d antioxidant activity of the leave s ofErechitites hieracifolia by DPPH (1,1- diphenyl -2-picrylhydrazyl) radical scavenging method. Electrophorectic isoenzyme banding patterns of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were observed by native PAGE. The correlation of PPO and antioxidant enzymes is not investigated yet. That is need to further study.