한방 소갈약 추출물의 혼합물에 의한 항돌연변이 효과

Antimutagenic Effect of the Extract Complex of Korean Anti-thirst Herb Medicines

The three crude drugs of the Kalopanax pictus(Araliaceae) roots (K), Pueraria thunbergiana(Leguminosae) flowers (P), and the Rhus verniciflua(Anacardiaceae) heartwood (R) used for anti-thirst drugs in oriental herb medicine were extracted with MeOH, respectively, and the successive fractionation of the extract gave EtOAc extract. Certain amount ratios of the three extracts were also prepared to compare the antimutagenicity in Ames test. In N- methyl-N(-nitro-N- nitrosoguanidine (MNNG; 0.4 (g/plate)-induced test, the activities of complex mixture were observed between the highest antimutagenic activity of K extract and the lowest P extract. In aflatoxin (AFBI)-induced test, the EtOAc complex (K : p : R=1 : 1 : 3) labeled I-113 decreased the revertants of Salmonella typhimurium TA100 by 95%, which activity were highest among other extracts or complexes mixture used. Fractionation of organic solvent mostly increased the anti-mutagenicity. These trends were also observed in the antimutagenicity test of the mixture of each active component of kalopanaxsaponin A, tectorigenin and sulfuretin. These results supported that many kinds of anti-thirst herb medicine in the prescription could effectively prevent cancer disease.